Phosphate-buffered saline ( PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH. The osmolarity and ion concentrations of the solutions match those of the human body (isotonic).
Application of phosphate buffered saline (PBS):
PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing. PBS with EDTA is also used to disengage attached and clumped cells. Divalent metals such as zinc, however, cannot be added as this will result in precipitation. For these types of applications, Good’s buffers are recommended. PBS has been shown to be an acceptable alternative to viral transport medium regarding transport and storage of RNA viruses, such as SARS-CoV-2.
By creating a strong chemical bond, bioactive glasses (bioglass) show a suitable biological response in the common season of bone and tissue, and therefore have wide applications in medicine. However, due to the not so favorable mechanical properties of these glasses, their use in applications under load faces problems, while metal alloys are still the best option for use in places that bear mechanical load.
Tricalcium phosphate, known as TCP, is a calcium salt of phosphoric acid with the chemical formula Ca3(PO4)2. Tricalcium phosphate is a white solid with low solubility, and most commercial samples are actually very similar to hydroxyapatite. Calcium is an essential mineral that is necessary for many body functions and especially the development of bones. We all need to get a certain amount of this mineral through our diet every day to maintain good health.
Simulated body solution (SBF) is a solution with an ion concentration relatively similar to blood plasma, which is prepared under physiological body temperature and pH conditions. This solution was first used by Kokobo and his colleagues to investigate the changes on the surfaces of bioactive glass ceramics. This test is one of the common tests for investigating the bioactivity or biodegradability of biomaterials, and due to the ease of work and the lack of equipment and facilities, it has a wide range and high application, and at the same time, it provides decisive results for researches. has it.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. It is a water-based saline solution that contains sodium hydrogen phosphate, sodium chloride, and in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to stabilize the pH. The concentration of osmolarity and ion concentration of this solution corresponds to the environment of the human body.
Chitosan is composed of repeating units of chitin, represented by the chemical formula C6H11NO4. Chitin is a natural mucopolysaccharide that is abundantly found in the exoskeleton of arthropods such as shrimp and crab and the cuticle of insects. Chitosan is obtained from the deacetylation of chitin, so that by adding concentrated sodium hydroxide solution and heat, the acetylamine groups in chitin are converted to amine agents.
